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Plasmid#204694PurposeThis plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 and NUTPromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
UseTagsmNeonGreen2 on ORF1pExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
Plasmid#196974PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and mNeonGreenExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown
Plasmid#188634PurposeHDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFRDepositorInsertEGFR (EGFR Synthetic, Human)
UseCRISPRTagsCD3ε-mNeonGreenExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen. PGK-puro
Plasmid#136255PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH
Plasmid#182425PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp) and Vesicle Nucl…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT-mRuby3
Plasmid#179226Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 HGV-mRuby3
Plasmid#179227Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreen and mRuby3ExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
Plasmid#173453PurposeContains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)
Plasmid#173455PurposeContains mScarlet-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300
Plasmid#204697PurposeThis plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 to p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332)-mNeonGreen
Plasmid#174714PurposeStable expression of fluorescent Separase cleavage sensor with human MeikinDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332PromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept
Plasmid#182424PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp), and Vesicle Nuc…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeongreen-pGBD(PKN1 codon optimized)
Plasmid#129634PurposeVisualization of active RhoADepositorInsertRhoA binding doamin of PKN1(30 - 100) codon optimized
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
Plasmid#183744PurposeExpresses Ki67 (shuffled Nterm)-mNeonGreenDepositorInsertMKI67 (shuffled Nterm) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationCD domain with shuffled 4 fragments fused to the …PromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only