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Showing: 281 - 300 of 935 results

  1. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  2. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

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  3. pTJK482

    Plasmid
    #138001
    Purpose
    GATA1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    GATA1 (GATA1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138001-plasmid-map-sequence-id-274995

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  4. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

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  5. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  7. pCCl-MCS_miniCMV_miRFP703

    Plasmid
    #134985
    Purpose
    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector
    Depositor
    Claudio Mussolino
    Article
    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d
    Insert
    miRFP703
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    minCMV
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134985-plasmid-map-sequence-id-265014

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  8. pCCl-MCS_miniCMV_TagBFP

    Plasmid
    #134986
    Purpose
    The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector
    Depositor
    Claudio Mussolino
    Article
    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d
    Insert
    TagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    minCMV
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134986-plasmid-map-sequence-id-265016

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  15. pLenti-AncBE4max

    Plasmid
    #174164
    Purpose
    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.
    Depositor
    Wensheng Wei
    Article
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Insert
    AncBE4max
    Use
    Lentiviral
    Tags
    P2A-mCherry
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174164-plasmid-map-sequence-id-342437

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  16. pPRIME-TET-GFP-FF3

    Plasmid
    #11662
    Purpose
    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
    Depositor
    Stephen Elledge
    Article
    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1
    Insert
    FF3
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    11662-plasmid-map-sequence-id-7008

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  18. pPRIME-CMV-dsRed-FF3

    Plasmid
    #11664
    Purpose
    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase
    Depositor
    Stephen Elledge
    Article
    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1
    Insert
    FF3
    Use
    Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 1, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 281 - 300 of 935 results