3xHRE-MinTK-CRE
(Plasmid
#141143)
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Purposelentiviral expression vector to generate a hypoxia fate-mapping system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRLSIN.cPPT.PGK-GFP.WPRE
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Backbone manufacturerDidier Trono (Addgene plasmid # 12252)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xHRE-MinTK-CRE
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xHRE-MinTK-CRE was a gift from Daniele Gilkes (Addgene plasmid # 141143 ; http://n2t.net/addgene:141143 ; RRID:Addgene_141143) -
For your References section:
Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis. Godet I, Shin YJ, Ju JA, Ye IC, Wang G, Gilkes DM. Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1. 10.1038/s41467-019-12412-1 PubMed 31649238