Showing: 281 - 300 of 4623 results
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Plasmid#185915PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
R777-E111 Hs.IRS2
Plasmid#70395PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)DepositorInsertIRS2 (IRS2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R777-E112 Hs.IRS2-nostop
Plasmid#70396PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)DepositorInsertIRS2 (IRS2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSaCas9_GFP
Plasmid#64709PurposeCo-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cellsDepositorInsertSaCas9-2A-GFP
UseCRISPRTags2A-GFP and 3xFLAG-SV40 NLSExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECitrine-Hygro
Plasmid#44646DepositorInsertyECitrine
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECFP-Hygro
Plasmid#44647DepositorInsertyEmCFP
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
hSyn ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85844PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorInsertArcLightCo-Q239
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterhSyn1AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
UseTagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEPAGFP-CaUra3
Plasmid#44649DepositorInsertyEPAGFP
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
Cas9 MDC123
Plasmid#59184Purpose2x35S promoting Glycing Max codon optimized Cas9 with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoter2X35SAvailabilityAcademic Institutions and Nonprofits only -
G10 Cas9 MDC123
Plasmid#59187PurposeG10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoterG10AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJM143
Plasmid#134166PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pJM147
Plasmid#134167PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 281 - 300 of 4623 results