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HR_G3BP1-V5-APEX2-GFP
Plasmid#105284Purposehomologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
UseHuman homologous recombination donorTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHREAC
Plasmid#134908PurposeOverexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterdouble 35SAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST pcDNA5-FRT/TO-eGFP-Rif1
Plasmid#52506Purposemammalian expression vector of Rif1 siRNA resistantDepositorInsertRIF1 (RIF1 Human)
UseTagseGFPExpressionMammalianMutationsiRNA resistant sequence: ATAGTACPromoterAvailable sinceJune 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)
Plasmid#140005PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KIT-V5/HIS
Plasmid#201986Purposeexpression of human KIT receptor tyrosine kinase in mammalian cellsDepositorInsertKIT receptor tyrosine kinase (KIT Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-Low-CAR_RHL001
Plasmid#164824PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-Low CARDepositorInsertAntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJOE_pvdJ
Plasmid#110142PurposeSuicide plasmid with repair template for knockout in P. putidaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOM20-ddGFP-B
Plasmid#40291DepositorInsertTOMM20 (TOMM20 Human)
UseTagsddGFP-BExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-Caspase 1
Plasmid#41552DepositorInsertCaspase-1 (CASP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV immediate-early enhancer/promoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-TAX1BP1
Plasmid#175761PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_dTomato_nlsdTomato
Plasmid#213829PurposeAAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomatoDepositorInsertdTomato_P2A_nls.dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2241
Plasmid#141985PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA3 (GATA3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0091
Plasmid#142457PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-2P-Kv-WPRE (AAV9)
Viral Prep#179463-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-JEDI-2P-Kv-WPRE (#179463). In addition to the viral particles, you will also receive purified pAAV-hSyn-JEDI-2P-Kv-WPRE plasmid DNA. hSyn-driven expression (soma and AIS-localized) of the genetically encoded voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRIMPkit
Plasmid Kit#1000000225PurposeThe CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.DepositorAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only
