Addgene

We narrowed to 5,521 results for: #16

Showing: 2961 - 2980 of 5521 results

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pnEA-NvM-SpEdc1opt_155-180-GB1_AD

Plasmid

#148544

Purpose

Bacterial Expression of SpEDC1opt_155-180

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpEDC1opt_155-180

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSWD95

Plasmid

#158617

Purpose

CckA FRET Sensor

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-AlpJ

Plasmid

#139598

Purpose

expresses AlpJ in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

alpJ

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinO1

Plasmid

#139597

Purpose

expresses KinO1 in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

kinO1

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-UBC16

Plasmid

#104681

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC16 (UBC16 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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YCe2030 HC_Kan_bGHpA_p16

Plasmid

#100688

Purpose

part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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ZAK (ZAKA-c057)

Plasmid

#98225

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ZAKA (MAP3K20 Human)

Use

Baculovirus

Tags

His-6-TEV

Expression

Mutation

pfam00069:Pkinase 16-257

Promoter

polyhedrin

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGP4GY-cccdB

Plasmid

#83878

Purpose

Destination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCas73

Plasmid

#82398

Purpose

Δccm:Km, used to delete ccm operon

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

ccm deletion

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBW100ParsR

Plasmid

#78635

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBW205J105-gfp

Plasmid

#78644

Purpose

pSB3K3 carrying J105-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J105-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW203J116-gfp

Plasmid

#78642

Purpose

pSB3K3 carrying J116-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW202J114-gfp

Plasmid

#78641

Purpose

pSB3K3 carrying J114-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J114-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B11-Leum

Plasmid

#44319

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B11-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B11-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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