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Plasmid#113626PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomologyDepositorInsert7i sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
UseTagsE6 tagExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF-CjPglB-LpxE
Plasmid#128389PurposeExpression of C. jejuni PglB and F. tularensis LpxE in E. coliDepositorInsertsPglB oligosaccharylktransferase
LpxE phosphatase
UseTagsFlag tagExpressionBacterialMutationPromoterPtac and pBADAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-I-SceI-Trex2
Plasmid#44024DepositorInsertI-SceI fused to mTREX2
UseTagsNLS-HAExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAL-Aur1-2GFP-Hygromycin
Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG:: mCherry-2a-hM4Dnrxn
Plasmid#52523PurposeCo-expresses hM4Dnrxn and a red fluorescent labelDepositorInsertmCherry-2a-hM4Dnrxn
UseTags2xHAExpressionMammalianMutationPromoterCAgAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2+Nterm Spe1 +Cterm Age1 site
Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_231
Plasmid#91120PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIR Y1158,1162,1163F
Plasmid#24054DepositorInsertInsulin Receptor (INSR Human)
UseTagsExpressionMammalianMutationY1158F, Y1162F, Y1163FPromoterAvailable sinceDec. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ
Plasmid#192387PurposeTo act as the 0-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_H2B_MS2
Plasmid#81082PurposeTranslation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_H2B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLQ-KO-tgt50
Plasmid#126578PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-Pspac-donor-tgt50
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
UseTags8xHisExpressionBacterialMutationPromoterPBAD30Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only