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We narrowed to 19,367 results for: pET

Showing: 2941 - 2960 of 19367 results

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pET51b(+)_LAMA-N95 (for GFP)

Plasmid

#130716

Purpose

Plasmid encoding the bacterial expression of LAMA-N95 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-N95

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET 15b-UbcH5c/2418

Plasmid

#11473

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5c (UBE2D3 Human)

Use

Tags

Expression

Bacterial

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pET21a(+) LdtMt2 (131-408)

Plasmid

#59337

Purpose

Expresses LdtMt2 for L,D-transpeptidation

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

LdtMt2 (131-408)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pET11a-CA(A14C, E45C, A92E)

Plasmid

#79046

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C, A92E)

Use

Tags

Expression

Bacterial

Mutation

A14C, E45C, A92E mutations in HIV-1 CA protein

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hisx6-Sirt6-H133Y

Plasmid

#20279

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Histidine 133 to Tyrosine

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pET28b(+)_S. cerevisiae Hbs1(1-611)

Plasmid

#39336

Depositor

SeWon Suh

Insert

Elongation factor 1 alpha-like protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-11a (GB1 WT - A34F)

Plasmid

#136609

Purpose

Expresses GB1 WT mutant (A34F)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT mutant (A34F)

Use

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F

Promoter

T7 promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b/Shigella flexneri IpaA

Plasmid

#46178

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

IpaA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Use

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV-AICD

Plasmid

#69927

Purpose

bacterial expression of MBP-TEV-AICD

Depositor

Thomas Wassmer, Zita Balklava

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

47 aa long

Promoter

T7

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET-TALE-mCherry-15b

Plasmid

#138287

Purpose

Express tTALE-mCherry

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-mCherry

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Galectin-8 linker deletion

Plasmid

#68806

Purpose

Express Galectin-8 linker deletion protein in Bacterial cells

Depositor

Hyun Kyu Song

Article

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Use

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKsubDD-YPet-NES

Plasmid

#84633

Purpose

Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsubDD-YPet-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-DEUT RUVBL1 Delete

Plasmid

#159144

Purpose

Expression and protien purification of mutated RUVBL1

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL1 (RUVBL1 Human)

Use

Tags

Expression

Bacterial

Mutation

amino acid 1-463, Glu134-Glu237 mutated to GPPG

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-U

Plasmid

#190969

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-U

Plasmid

#190973

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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