pET15b/Shigella flexneri IpaA
(Plasmid
#46178)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerEMD
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 7607
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIpaA
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SpeciesShigella flexneri
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Insert Size (bp)1902
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IpaA cDNA was amplified from pEC14, supplied by M. Venketesan (Walter Reed Army Institute of Research, Washington, DC),
using appropriate primers to introduce an NdeI site before the initiating ATG and an XhoI site downstream of the stop codon. The
PCR product was transformed into TOPO pCRII (Invitrogen). The NdeI and XhoI fragment of IpaA cDNA was isolated from a pCRII clone containing the correct sequence and inserted into pET15b vector (Novogen) to generate pET15b/IpaA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b/Shigella flexneri IpaA was a gift from Susan Craig (Addgene plasmid # 46178 ; http://n2t.net/addgene:46178 ; RRID:Addgene_46178) -
For your References section:
Spatial distribution and functional significance of activated vinculin in living cells. Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW. J Cell Biol. 2005 May 9;169(3):459-70. 10.1083/jcb.200410100 PubMed 15883197