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Plasmid#35137DepositorInsertHPV2a E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6656 MSCV-P C-FlagHA 25E7-Kozak
Plasmid#35022DepositorInsertHPV25 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra2
Plasmid#179477PurposeER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
882 pSG5L HA PTEN G129E
Plasmid#10746DepositorInsertPTEN (PTEN Human)
UseTagsExpressionMammalianMutationG129EPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-Electra1-P2A-mScarlet
Plasmid#196113Purposezebrafish expression of nbt-Electra1-P2A-mScarletDepositorInsertnbt-Electra1-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorInsertGOS1 (GOS1 Budding Yeast)
UseTagsceruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
817 pSG5L HA PTEN G129R
Plasmid#10748DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationG129RPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-GW
Plasmid#194634PurposeGateway destination vector for mammalian expression of Virotrap bait constructsDepositorInsertp55 GAG
UseTagsPQS1ExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2pA2-drl:APEX2-mCherry
Plasmid#188945PurposePlasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoterDepositorInsertdrl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
UseTol2 destination vector (attr4-r3)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherry-hyBRET-ERK-NLS
Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-nlstdTomato
Plasmid#132985Purposefull length of nuclear localized tdTomato in pCS expression vectorDepositorInserttdTomato
UseTagsNLS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mTagBFP2
Plasmid#184940PurposeER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-mTagBFP2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only