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Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MCU-Flag-TetOFF
Plasmid#185657PurposeTet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporterDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52379PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsGFP-2A-puro
OCT4
UseTagsExpressionMammalianMutationPromoterOCT4Available sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM3
Plasmid#179957PurposeExpression vector for human IFITM3.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM1
Plasmid#179955PurposeExpression vector for human IFITM1.DepositorInsertIFITM1 (IFITM1 Human)
UseTagsExpressionMammalianMutationP13APromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 puro - CRBN (#1)
Plasmid#166240PurposesgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1DepositorInsertCRBN (CRBN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 puro - CRBN (#2)
Plasmid#166241PurposesgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1DepositorInsertCRBN (CRBN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Promoter-hPE(+)-Luc
Plasmid#172003PurposeLuciferase Reporter for Human PTEN Enhancer PE - Forward OrientationDepositorInsertHuman PE sequence (hPE)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoterSV40 PromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BMI1-Ubc-rtTA
Plasmid#198191PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBMI1 (BMI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
scAAV-Cre
Plasmid#177933PurposeExpresses Cre-recombinase and can be packaged into AAV particles.DepositorInsertCAG-Cre
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
scAAV-sgRNA-GFP
Plasmid#177935PurposeExpresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic miceDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse Oct4-GFP GOF18 transgenic reporter
Plasmid#60527PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)DepositorInsertGFPpA
UseTagsExpressionMammalianMutationPromoterOct4Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Acly(S455A)
Plasmid#70767PurposeExpresses phosphomutant mouse ATP-citrate lyase in mammalian cellsDepositorInsertATP Citrate Lyase (Acly Mouse)
UseTagsmyc-epitopeExpressionMammalianMutationS455APromoterEF1alphaAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM2
Plasmid#179956PurposeExpression vector for human IFITM2.DepositorInsertIFITM2 (IFITM2 Human)
UseTagsExpressionMammalianMutationA33V, T41M, V121IPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
FOR human OCT4 TALEN
Plasmid#52412PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD NG NN NN NN HD NG HD NG HD HD HD NI NG
UseTALENTagsExpressionMutationPromoterCAGGSAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCU-mCerulean
Plasmid#185809PurposeHuman mitochondrial calcium uniporter with C-terminus mCerulean tagDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only