Addgene

We narrowed to 24,567 results for: sta

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pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDT-sgRNA

Plasmid

#138271

Purpose

Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

sgBG, sgTS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-Flt3L

Plasmid

#174600

Purpose

Secretes the extracellular domain of murine Flt3L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secretes extracellular domain of mouse Flt3-L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN HAYFP-LOV24

Plasmid

#49570

Purpose

regulate protein stability with light

Depositor

Thomas Wandless

Article

Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

Insert

YFP-LOV24

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

delete the last 3 aa from AsLOV2, with I532A muta…

Promoter

LTR

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Tags

Expression

Mutation

Promoter

GAPDH

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

Plasmid

#204497

Purpose

Expresses rtTA and Neomycin resistance genes in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

rtTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pWPI-TBX2

Plasmid

#185526

Purpose

Constitutive overexpression of human TBX2

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

TBX2 (TBX2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

pPhlF

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAd-Klf4

Plasmid

#19770

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Science. 2008 Sep 25. ():.

Insert

Klf4 (Klf4 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
AIRAP 9: PSMD2.GST.V2.pcDNA3

Plasmid

#21799

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD2 (PSMD2 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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CyTERM-mGFP

Plasmid

#62237

Purpose

ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

Depositor

Erik Snapp

Article

Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

Insert

CyTERM-mGFP

Use

Tags

mGFP

Expression

Mammalian

Mutation

signal anchor of rabbit cytochrome p450 and monom…

Promoter

CMV

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-myc T58A

Plasmid

#19763

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMP71-IFN-gamma

Plasmid

#174602

Purpose

Constitutive expression of secreted murine IFN gamma

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Constitutive expression of secreted murine IFN gamma

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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