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Plasmid#181834PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).DepositorInsertLyn-dInPAkt
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR
Plasmid#181832PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).DepositorInsertLyn-PlcR
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR(K30/32L)
Plasmid#181833PurposePhospholipid binding-deficient Lyn-dPlcR negative-control plasmid.DepositorInsertLyn-dPlcR (K30L,K32L)
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1
Plasmid#188567Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp1
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-Fis1
Plasmid#182576PurposeExpresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertLifeAct-GFP-Fis1
UseTagsFis1 and LifeActExpressionMammalianMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FLAG
Plasmid#60360PurposePlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsEGFP and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hATMS1981A
Plasmid#32300DepositorInsertATM (ATM Human)
UseTagsFlag and HisExpressionMammalianMutationS1981APromoterAvailable sinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
UseTagsExpressionYeastMutationPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-mCherry
Plasmid#45147DepositorInsertLAMP1-mCherry (LAMP1 Synthetic, Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-GFP-RGG
Plasmid#124930PurposeExpression of RGG-GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-WT
Plasmid#111575PurposeExpresses full length PARP1 in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti EF1a GFP-mCherry-mPlin1
Plasmid#189005PurposeFluorescent reportor for lipophagyDepositorInsertGFP, mChery, Plin1 (Plin1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Antares
Plasmid#208852PurposeExpresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.DepositorInsertAntares
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synp-F-H2B-jRGECO1a
Plasmid#102995PurposeExpresses histone fused jRGECO1a under the synapsin promoterDepositorInsertH2B-jRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only