Addgene

We narrowed to 61,805 results for: kan

Showing: 2921 - 2940 of 61805 results

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mEos2-Alpha-Actinin-C-14

Plasmid

#57347

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac-His-Flag-Tipin-L195A

Plasmid

#23120

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag- and His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin-L195A

Plasmid

#23121

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Combined γB2FL-FRET probe

Plasmid

#209094

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP12-talinC11-ABS3mut-mEmerald

Plasmid

#202383

Purpose

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2S31A

Plasmid

#174332

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-FokI-dCas9-EGFP-L2

Plasmid

#141047

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-FokI-dCas9-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Flag-Tipin-L195A

Plasmid

#23118

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
DHC1-AID-Clover donor (Hygro)

Plasmid

#158622

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK297 (DHC1-mAID Hygro)

Plasmid

#140542

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK296 (DHC1-mAID Neo)

Plasmid

#140541

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGG442

Plasmid

#165616

Purpose

Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t

Use

CRISPR

Tags

3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA7 Neo

Plasmid

#140543

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Neo)

Plasmid

#158621

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-Actin-7

Plasmid

#57339

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Actin (ACTB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits