Addgene

We narrowed to 4,629 results for: codon optimized

Showing: 2921 - 2940 of 4629 results

Results per page:

pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM4 (G470A/G471N)

Plasmid

#154859

Purpose

Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM4 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Glycine 470 changed to Alanine and Glycine 471 ch…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM1 (A144G/N145G)

Plasmid

#154873

Purpose

Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM1 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Alanine 144 changed to Glycine and Asparagine 145…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCW57.1 ISCU D71A

Plasmid

#169880

Purpose

Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative)

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

ISCU

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon Optimized, D71A iron-sulfur cluster trappin…

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Use

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRWJB004

Plasmid

#160112

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

UBQ10

Availability

Academic Institutions and Nonprofits only

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pRWJB006

Plasmid

#160113

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Availability

Academic Institutions and Nonprofits only

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prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)-Strep

Plasmid

#164444

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)

Use

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine

Promoter

Availability

Academic Institutions and Nonprofits only

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pUPD2 AsCas12a (GB1438)

Plasmid

#160572

Purpose

Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AsCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Availability

Academic Institutions and Nonprofits only

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pUPD2 LbCas12a (GB1439)

Plasmid

#160573

Purpose

Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

LbCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

Plasmid

#160635

Purpose

TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

AcrIIA4

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Availability

Academic Institutions and Nonprofits only

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pAT15547_PEmax-SuperFi-Cas9

Plasmid

#184377

Purpose

Mammalian expression of SuperFi-Cas9 PEmax prime editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

PEmax-SuperFi-Cas9

Use

CRISPR

Tags

SV40 NLS and c-myc NLS

Expression

Mammalian

Mutation

H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkaš et al Engineering Microbiology, 2022

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0001

Plasmid

#185625

Purpose

MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0202

Plasmid

#185626

Purpose

MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2+ heiCas9

Plasmid

#186929

Purpose

For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

Depositor

Joachim Wittbrodt

Article

Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

Insert

myc-flexible linker-oNLS-Cas9-oNLS

Use

CRISPR

Tags

myc-flexible linker-optimizedNLS and optimizedNLS

Expression

Mammalian

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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