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Plasmid#30371DepositorInsertPTEN-S10N (PTEN Human)
UseTagsExpressionMammalianMutationS10NPromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-PTEN-T383D
Plasmid#30381DepositorInsertPTEN-T383D (PTEN Human)
UseTagsExpressionMammalianMutationT383DPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-S75F
Plasmid#24574DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationserine 75 changed to phenylalanine (codon TCT cha…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-A80T
Plasmid#24575DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationAlanine 80 changed to threonine (codon GCA to ACA)PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-G139S
Plasmid#24576DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationGlycine 139 changed to serine (codon GGT to AGT)PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-S437R
Plasmid#24577DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationSerine 437 changed to Arginine (codon AGT to CGT)…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Halo-KDEL
Plasmid#124316PurposeExpresses ER specific HaloTag proteinDepositorInsertHalo tag
UseTagsKDEL SeqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR
Plasmid#181830PurposeGenetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.DepositorInsertLyn-PlcR
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1-REX-YFP
Plasmid#48247PurposeGenetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertREX-YFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
UseTagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-KIRIN1
Plasmid#107416PurposeMammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequenceDepositorInsertKIRIN1
UseTagsNuclear exclusion sequence (NES) (LALKLAGLDIGS)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFAM-SNAPf
Plasmid#187909PurposeMammalian cell expression of SNAPf-labeled TFAMDepositorInsertTFAM (TFAM Human)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only