Addgene

We narrowed to 3,812 results for: biorxiv

Showing: 2901 - 2920 of 3812 results

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pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188746

Purpose

Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

nonTATAchrX

Availability

Academic Institutions and Nonprofits only

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pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188747

Purpose

Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Availability

Academic Institutions and Nonprofits only

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pMV1 pEF1a-HGF

Plasmid

#188748

Purpose

Vector constitutively expressing HGF

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

HGF (HGF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Availability

Academic Institutions and Nonprofits only

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pMV2 AAV-RapaInducible-NFZ-HGF

Plasmid

#188749

Purpose

Rapamycin inducible AAV vector expressing HGF

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

HGF (HGF Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Rapamycin inducible

Availability

Academic Institutions and Nonprofits only

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pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

Plasmid

#188758

Purpose

Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

VP64

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hU6 and hUbC

Availability

Academic Institutions and Nonprofits only

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pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

Plasmid

#190755

Purpose

Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Inserts

GNB1 (GNB1 Human)
GNG2 (GNG2 Human)
GNAI1 (GNAI1 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLV-CS-193

Plasmid

#197137

Purpose

CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

mCherry(M1V/M9A)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSI-218

Plasmid

#197139

Purpose

All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

CRISPR

Tags

SpCas9-PmCDA1-UGI

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

Plasmid

#195537

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Availability

Academic Institutions and Nonprofits only

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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#195542

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Availability

Academic Institutions and Nonprofits only

Enlarge