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Plasmid#215674PurposeSplit hygromycinR landing pad insertion plasmid for ChrIIIDepositorInsert5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
UseCRISPR and Cre/LoxTagsExpressionWormMutation3' ∆HYGR is promoterless and encodes aa60-341PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4(1-147)
Plasmid#195029PurposeThe control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vectorDepositorInsertGAL4 (1-147)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 Prg4 promoter-luciferase
Plasmid#195034PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-hArfaptin1
Plasmid#206402PurposeMammalian expression of hArfaptin1DepositorInsertArfaptin1 (ARFIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.040
Plasmid#184974PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
UseTagsExpressionYeastMutationADE2 donor P272stopPromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBN216
Plasmid#167963Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN217
Plasmid#167964Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN262
Plasmid#167967Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
UseTagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
UseTagshexa-histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN202
Plasmid#167950Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-MKP-3 C293S
Plasmid#131525PurposeExpresses MKP-3 with C293S mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Cysteine 293 to SerinePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT3
Plasmid#118985PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT5
Plasmid#118987PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationmutK2-mutSP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT7
Plasmid#118989PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-mutK1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-KD
Plasmid#114066Purposeprotein productionDepositorInsertMps1-KD (TTK Human)
UseTagsGSTExpressionInsectMutationMps1 KD (D664A)PromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc
Plasmid#91935PurposeBacterial expression of maize SUMO1a and inactive SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc
Plasmid#91933PurposeBacterial expression of maize SUMO1a and inactive SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only