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Plasmid#128999PurposepZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP-VPS21
Plasmid#105265PurposePlasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.DepositorInsertVps21 (VPS21 Budding Yeast)
UseTagsmsGFPExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1
Plasmid#41606DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsTRP1 reverse orientationExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5V1
Plasmid#165058PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFPDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE GFP
Plasmid#32120DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303PromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 WT
Plasmid#87288PurposeEncodes Y2H Gal4 activation domain for Hsh155 WTDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3
Plasmid#112658PurposeExpression S.cerevisiaeDepositorInsertsfGFP-Glo3
UseTagssfGFPExpressionYeastMutationPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP9
Plasmid#87593PurposeEncodes Y2H Gal4 DNA binding domain for Prp9DepositorInsertPRP9 (PRP9 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGBKT7-PRP43
Plasmid#87596PurposeEncodes Y2H Gal4 DNA binding domain for Prp43DepositorInsertPRP43 (PRP43 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BIO-hphMX4
Plasmid#26880DepositorInsertBIO-tag
UsePcr-tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A GFP
Plasmid#32125DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP, HDEL, and Kar2ExpressionYeastMutationK108APromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP5
Plasmid#87592PurposeEncodes Y2H Gal4 DNA binding domain for Prp5DepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET-T2A-mTurquoise2
Plasmid#118444PurposeProduces equimolar expression of ymYPET and mTq2DepositorInsertymYPET-T2A-mTurquoise2
UseTagsExpressionYeastMutationYPET A206K, F208S, E232L, N235DPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 Y292C
Plasmid#87291PurposeEncodes Y2H Gal4 activation domain for Hsh155 Y292CDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationY292CPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK3F-PYK1
Plasmid#85771PurposeN-ICE plasmid pK3F-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-T|C-iGFP-RCY1
Plasmid#105281PurposeOverexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.DepositorInsertRcy1 (RCY1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only