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Plasmid#136985PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activityDepositorInsertHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
UseTagsExpressionMammalianMutationcarries D110E mutation in the reverse transcripta…PromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGarnet2-RITA
Plasmid#104314Purposecodes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cellsDepositorInsertmGarnet2-RITA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-tdTomado-tDeg
Plasmid#185402PurposetdTomado-tDeg fluorogenic proteinDepositorInserttdTomado-tDeg
UseTagsExpressionMammalianMutationWTPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER2-LSSmGFP
Plasmid#186538PurposeLocalization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiencyDepositorInsertLSSmGFP
UseTagsCalreticulin and KDELExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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ER2-mGreenLantern
Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm4b-pA
Plasmid#104486PurposeExpress mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.DepositorInsertKdm4b (Kdm4b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP1-10_TEV
Plasmid#81242PurposeZipped GFP1-10 with TEV cleavage sequence T2A mCherryDepositorInsertzipped GFP11 with TEV cleavage sequence T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
TNXXL-CTS20
Plasmid#48342PurposeFRET reporter for Ca2+ inside primary ciliaDepositorInsertCTS20
UseTagsTN-XXLExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Integrin-αv Ectodomain M400C
Plasmid#159642PurposeContains integrin alpha v residues 1–960 with the M400C mutationDepositorInsertIntegrin alpha v ectodomain
UseTagsExpressionMammalianMutationM400CPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RFP-SZ2
Plasmid#124936PurposeExpression of RFP-SZ2DepositorInsertRFP-SYNZIP2
UseTagsmCherryExpressionMammalianMutationSZ2 domainPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670nano3-NbGFP
Plasmid#184686PurposeNanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-NbGFP
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_ScPH1-S
Plasmid#131193PurposeExpresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sitesDepositorInsertScPH1-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS9
Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS9-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) Tacδ
Plasmid#81177PurposeMammalian expression of human Tacδ (GFFKR cytoplasmic tail)DepositorInsertTacδ
UseTagsKLGFFKRExpressionMammalianMutationCD25 amino acids 1 to 263 fused to KLGFFKR cytopl…PromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 31-52
Plasmid#35179DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationDeletion of C-terminal domain repeats 1-30PromoterCMVAvailable sinceOct. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 1-22
Plasmid#35178DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationDeletion of C-terminal domain repeats 23-52PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tubulin.K40Q
Plasmid#32912DepositorInsertTubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40QPromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minSV40-sfGFP-CMV_dsRed
Plasmid#117400PurposesfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
VAChT-pHuji
Plasmid#105319PurposeVAChT tagged with pH-sensitive red fluorescent protein pHujiDepositorInsertVAChT (Slc18a3 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only