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Showing: 2881 - 2900 of 13821 results

  5. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

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  7. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Use
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  11. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  15. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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  20. pET24a-VHH-tev-APEX2

    Plasmid
    #117751
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117751-plasmid-map-sequence-id-228349

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Showing: 2881 - 2900 of 13821 results