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Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD2 HRD
Plasmid#207078PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS
Plasmid#217478PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1sDepositorInsertNier1s
UseTags3xNLS, P2A-EGFP-3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS
Plasmid#217479PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1bDepositorInsertNier1b
UseTags3xNLS, P2A-EGFP-3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-MiniR
Plasmid#208328PurposeMiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.DepositorInsertH2B-MiniR
UseTagsMiniRExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TinyERRE
Plasmid#208324PurposeC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TinyERRE
UseTagsTinyERREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TREER-Actin
Plasmid#208330PurposeTREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.DepositorInsertTREER-Actin
UseTagsTREERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-PuRRRE
Plasmid#208329PurposePuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.DepositorInsertTOMM20-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PunyEEER
Plasmid#208326PurposeC-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PunyEEER
UseTagsPunyEEERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-mCherryEA
Plasmid#207867PurposeMammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmCherryEA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.13_GagZip-MCP_Zip-P2A-eGFP
Plasmid#205528PurposeExpress HIV GagZip-MCP_Zip-P2A-eGFPDepositorInsertGagZip-MCP_Zip
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.7_Gag-dZF2-MCP-P2A-eGFP
Plasmid#205523PurposeExpress HIV Gag-delta-ZF2-MCP-P2A-eGFPDepositorInsertGag delta ZF2-MCP
UseTagsExpressionMammalianMutationZF2 deletedPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP
Plasmid#205529PurposeExpress HIV GagZip-dp1dp6-MCP-P2A-eGFPDepositorInsertGagZip-dp1dp6-MCP
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, p1 and…PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - pRSET
Plasmid#193947PurposeBacterial expression vector for a low-affinity calcium indicatorDepositorInsertGreenT-EC
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC.b - pRSET
Plasmid#193948PurposeBacterial expression vector for a low-affinity calcium indicatorDepositorInsertGreenT-EC.b
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC.c - pRSET
Plasmid#193949PurposeBacterial expression vector for a low-affinity calcium indicatorDepositorInsertGreenT-EC.c
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205544PurposeExpress EPN1-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP-I
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
UseTagsmCherry (K187N -please see depositor comments)ExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only