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pCDNA5/FRT/TO_TOMM20_HaloTag9
Plasmid#169335PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-DIO-BiPOLES-mCerulean
Plasmid#154949PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Mito-7
Plasmid#58115PurposeLocalization: Mitochondria, Excitation: 554, Emission: 581DepositorInsertMito (COX8A Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRA
Plasmid#131035PurposeExpresses OT-I TCRADepositorArticleInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG106 Lyn11-FAST
Plasmid#130721PurposeExpresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cellsDepositorInsertlyn11-FAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-24:MYH10-mEGFP
Plasmid#87428PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10DepositorInsertMYH10 Homology Arms with mEGFP-linker (MYH10 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr
Plasmid#19325DepositorArticleInsertPunc-122::GFP
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
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PKC gamma DN
Plasmid#21239DepositorInsertPKC gamma (Prkcg Mouse)
UseTagsHAExpressionMammalianMutationK380RPromoterAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHEFI-OptoCARCD19-mScarlet
Plasmid#170465PurposeExpresses an optically-controllable Chimeric antigen receptor that binds CD19 antigen, fused to mScarlet fluorophoreDepositorInsertOptoCAR-CD19
UseLentiviralTagsmScarletExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N1
Plasmid#125547PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to n-terminus of Gatew…ExpressionBacterial and MammalianMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-(3×FLAG)-TEV-ProtA
Plasmid#52692PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromotern/aAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH6
Plasmid#129424PurposeExpression human MSH6 protein using baculovirusDepositorInserthuman MSH6 (MSH6 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MP-84-spCas9-U6-gRNA
Plasmid#206935PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterMP-84Available sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RHPMP192 (pLifeAct sfGFP)
Plasmid#88843PurposeLocalization by Fluorescence microscopy of lifeAct fused to super folder GFPDepositorInsertLifeAct
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria1
Plasmid#124853PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only