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Plasmid#191764PurposeExpression of EGFP fused to core p300 histone acetyltransferaseDepositorInsertFRB-EGFP-p300 core (EP300 Human)
UseTagsEGFP (N-terminal of p300 core) and FRB (N-termina…ExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11'].DepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFR (del3) L747-E749del, A750P
Plasmid#11015PurposeRetroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cellsDepositorInsertEGFR (del3) (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL747-E749 deleted, A750PPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
EB3-mNeonGreen
Plasmid#191761PurposeExpression of mNeonGreen fused to EB3DepositorInsertEB3-mNeonGreen (MAPRE3 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p80-Katanin-mNeonGreen
Plasmid#191762PurposeExpression of mNeonGreen fused to p80-KataninDepositorInsertp80-Katanin-mNeonGreen (KATNB1 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralTagsExpressionMutation15 silent wobble mutations corresponding to three…PromoterSV40Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas-pML-1B_6xHis-Cas12m
Plasmid#192282PurposeMmCas12m fused to His-tag for protein purificationDepositorInsertMmCas12m
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp15
Plasmid#156481PurposeBacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tagDepositorInsertNsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
UseTagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp9
Plasmid#156478PurposeBacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tagDepositorInsertNsp9 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_CTD
Plasmid#156484PurposeBacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tagDepositorInsertN_CTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_NTD
Plasmid#156483PurposeBacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tagDepositorInsertN_NTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3d_Ub2_PLPro
Plasmid#156472PurposeBacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tagDepositorInsertNsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp1
Plasmid#156465PurposeBacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tagDepositorInsertNsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp4
Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp8
Plasmid#156477PurposeBacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tagDepositorInsertNsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only