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Plasmid#18879PurposeCaspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomatoDepositorInsertmAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
UseTagsExpressionMammalianMutationmAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…PromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3
Plasmid#213973PurposeAAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locusDepositorInsertShank3 sgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CVS-G-WPRE-pGHpA
Plasmid#67528Purpose"Expresses the rabies glycoprotein"DepositorInsertRabies virus strain CVS-11 glycoprotein (G)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GT
Plasmid#26198DepositorInsertEnhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
UseAAV and Cre/LoxTagsExpressionMutationTVA mammalian codon optimizedPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-GCaMP6s-WPRE-pGHpA
Plasmid#67526Purpose"Fluorescent reporter for calcium imaging"DepositorInsertGCaMP6s
UseAAVTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G88P3-HA-hM3Dq
Plasmid#213972PurposeAAV vector with G88P3 promoter that can drive robust gene expression in MSNsDepositorInsertG88P3
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124543PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA
Plasmid#124542PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41 C55GPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
IR25a-T2A-QF-AD
Plasmid#162526PurposePlasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a geneDepositorInsertIr25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-c
Plasmid#53078Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124541PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
UseTagsExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124539PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55A, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124545PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124544PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55A, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124540PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124538PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA
Plasmid#124537PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41 C55GPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-n
Plasmid#53077Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a::DIO-iLMO2
Plasmid#129482PurposeAAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only