Addgene

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Human Genome-wide Intron Tagging Library, Frame 0

Pooled Library

#216133

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
lentiCas9-Blast (Lentiviral Prep)

Viral Prep

#52962-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Promoter

EFS-NS

Tags

None

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
L5-LexAp65-L2

Plasmid

#41437

Depositor

Steve Stowers

Insert

LexAp65

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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L1-10XQUAS-L4

Plasmid

#41436

Depositor

Steve Stowers

Insert

10XQUAS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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L1-10XQUAS-R5

Plasmid

#41435

Depositor

Steve Stowers

Insert

10XQUAS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR L1-13XLexAop2-L4

Plasmid

#41434

Depositor

Steve Stowers

Insert

13XLexAop2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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R4-LexAp65-R3

Plasmid

#41438

Depositor

Steve Stowers

Insert

LexAp65

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR L1-13XLexAop2-R5

Plasmid

#41433

Depositor

Steve Stowers

Insert

13XLexAop2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Article

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Insert

NanoLuc (Nluc)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA+

Plasmid

#86014

Purpose

Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14-tRNA chimera (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Promoter

LPP and Ptac

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA-

Plasmid

#86015

Purpose

Multi-gene expression system with human box C/D proteins with U14

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14 (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Promoter

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pUltra-Plus

Plasmid

#161775

Purpose

3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

Depositor

Jorge Luis Arias-Arias

Type

Empty backbone

Use

Lentiviral; Multicistronic

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST-3x attB

Plasmid

#220329

Purpose

includes 3 standard UAS cassettes

Depositor

Erdem Bangi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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2xUAS-1xhsp70-attB

Plasmid

#220317

Purpose

includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette

Depositor

Erdem Bangi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJK531

Plasmid

#72512

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pUC119-MCS

Plasmid

#58807

Purpose

contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

Depositor

Jen Sheen

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJK539

Plasmid

#72515

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK549

Plasmid

#72520

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK555

Plasmid

#72521

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK577

Plasmid

#72528

Purpose

Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Use

Gene synthesis

Tags

Expression

Mutation

Promoter

n/a

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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