-
Plasmid#66880PurposeExpresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160S)
UseTagsnoneExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160E,HisTagDel)
Plasmid#66879PurposeExpresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160E)
UseTagsExpressionBacterialMutationS160EPromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_ITPKA
Plasmid#65689PurposeExpresses ITPKA-ONL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsONLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_PTS1
Plasmid#65699PurposeExpresses YNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_Golgi
Plasmid#65694PurposeExpresses Golgi-CNL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_TUBB5
Plasmid#65679PurposeExpresses beta-tubulin-CNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_Golgi
Plasmid#65693PurposeExpresses Golgi-YNL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_ITPKA
Plasmid#65687PurposeExpresses ITPKA-YNL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsYNLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_PTS1
Plasmid#65700PurposeExpresses CNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_PTS1
Plasmid#65701PurposeExpresses ONL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_Mito
Plasmid#65671PurposeExpresses Mito-ONL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Cx43-7
Plasmid#57603PurposeLocalization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCx43
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
Plasmid#52898PurposeYpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.DepositorInsertYpd*1-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationChanged Tryptophane 80 to AlaninePromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-mJP-4
Plasmid#79599PurposeTransient expressionDepositorInsertJunctophilin-4 (Jph4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK289 (mAID-mClover-NeoR)
Plasmid#72827PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Neo
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK290 (mAID-mClover-Hygro)
Plasmid#72828PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Hygro
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-mOXT-hM3Dq-mCherry-WPRE
Plasmid#70717PurposeExpresses DREADDs Gq receptor under oxytocin promoter controlDepositorInserthM3Dq-mCherry
UseAAVTagsmCherryExpressionMutationPromotermouse oxytocinAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK293 (mAID-mCherry2-Hygro)
Plasmid#72831PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorInsertmAID-mCherry2-Hygro
UseCRISPRTagsmAID-mCherry2ExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only