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pBABE-zeo (pBABE-bleo)

Plasmid

#1766

Purpose

Retroviral vector for cloning and expressing your gene of interest. Zeocin selection.

Depositor

Jay Morgenstern, Hartmut Land, Bob Weinberg

Article

Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):35

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSC

Plasmid

#46896

Purpose

expression of scrambled shRNA

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

scrambled

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pRK5F Smad2

Plasmid

#12623

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad2 (SMAD2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pMW#2

Plasmid

#13349

Depositor

Marian Walhout

Article

Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

Insert

HIS3 reporter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB wt

Plasmid

#19313

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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mOrange2-pBAD

Plasmid

#54531

Purpose

Localization: Protein Expression Vector , Excitation: 549, Emission: 565

Depositor

Michael Davidson, Roger Tsien, Nathan Shaner

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

6xHis and mOrange2

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag PGC1alpha S570A

Plasmid

#15988

Depositor

Morris Birnbaum

Article

Li et al Nature. 2007 Jun 21. 447(7147):1012-6.

Insert

PGC1 alpha (PPARGC1A Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S570A

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pMPRAv3:∆luc:∆xbaI

Plasmid

#109035

Purpose

MPRA v3 empty backbone

Depositor

Pardis Sabeti, Ryan Tewhey

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Article

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Use

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Promoter

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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S48I48 ELP

Plasmid

#68394

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S48I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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NPY-mApple

Plasmid

#83498

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Article

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Insert

NPY (NPY Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A

Plasmid

#24898

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-TagRFP-t35s

Plasmid

#118386

Purpose

Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p-TagRFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4-mCherry

Plasmid

#80269

Purpose

mammalian expression plasmid for Plk4-mCherry

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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