Addgene

We narrowed to 6,564 results for: ski

Showing: 2781 - 2800 of 6564 results

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pAAV-GFAP-eLACCO1.1

Plasmid

#168790

Purpose

Astrocyte expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLacco1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 CA-LRP6-GFP

Plasmid

#29682

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

LDL-receptor related protein 6 (LRP6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

extracellular region deleted

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pCEP4-CCR5

Plasmid

#98945

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Use

Tags

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEY48

Plasmid

#191052

Purpose

klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

klp-6 (klp-6 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY47

Plasmid

#191051

Purpose

gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-21 (gcy-21 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Use

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Ensconsin-18-mEos2

Plasmid

#99230

Purpose

Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

mEos2

Use

Tags

Ensconsin (MAP7)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEY15

Plasmid

#191021

Purpose

flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-1 (flp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7_dead_mt

Plasmid

#167267

Purpose

Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Use

Tags

His x10

Expression

Bacterial

Mutation

D106A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Use

Tags

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Use

Tags

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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GlycophorinE-CD4d3+4-bio

Plasmid

#73095

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin E (GYPE Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag hDPY30

Plasmid

#15554

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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