pAAV-GFAP-eLACCO1.1
(Plasmid
#168790)
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PurposeAstrocyte expression of fluorescent sensor for extracellular lactate
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeLacco1.1
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Insert Size (bp)1821
- Promoter GFAP
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Tags
/ Fusion Proteins
- GPI (C terminal on insert)
- ER export (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer GFAP
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byRobert Campbell
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GFAP-eLACCO1.1 was a gift from Marie-Eve Paquet (Addgene plasmid # 168790 ; http://n2t.net/addgene:168790 ; RRID:Addgene_168790) -
For your References section:
A genetically encoded fluorescent biosensor for extracellular L-lactate. Nasu Y, Murphy-Royal C, Wen Y, Haidey JN, Molina RS, Aggarwal A, Zhang S, Kamijo Y, Paquet ME, Podgorski K, Drobizhev M, Bains JS, Lemieux MJ, Gordon GR, Campbell RE. Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2. 10.1038/s41467-021-27332-2 PubMed 34873165