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Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsUseTags2xSTIIExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Syn-Archon1-KGC-GFP-ER2
Plasmid#115892PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal.DepositorHas ServiceAAV8InsertArchon1-KGC-EGFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterSynAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-4xInsulator (JP6)
Plasmid#99627PurposeIfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locusDepositorInsertGt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
iMb-Notch-Mosaic (IR99.40)
Plasmid#99749PurposeRosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genesDepositorInsertMbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA position E3 (GB2240)
Plasmid#160562PurposetRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInserttRNA-gRNA position E3 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-CITED(216-242)+CBPTAZ1(340-439)
Plasmid#189961PurposeCoexpression of CBP TAZ1 and CITED in E coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)
Plasmid#189963PurposeCoexpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationL243A, E245A, L246APromotert7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)
Plasmid#189962PurposeExpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationPromotert7Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Archon1-KGC-GFP-ER2
Plasmid#115890PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115893PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorHas ServiceAAV8InsertArchon1-KGC-EGFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterSynAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115891PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1α promoter (1.1kb short version)Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EcNGRfE
Bacterial Strain#48935PurposeBase line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)DepositorBacterial ResistanceSpectinomycinAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #2 (low: no promoter, p029)
Pooled Library#217421PurposescQers promoter control #2 (low: no promoter, p029)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #3 (intermediate: UBC promoter, p041)
Pooled Library#217422PurposescQers promoter control #3 (intermediate: UBC promoter, p041)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #4 (intermediate: Pgk1 promoter, p042)
Pooled Library#217423PurposescQers promoter control #4 (intermediate: Pgk1 promoter, p042)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #5 (high: EEF1A1 promoter, p028)
Pooled Library#217424PurposescQers promoter control #5 (high: EEF1A1 promoter, p028)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #1 (low: minimal promoter, p027)
Pooled Library#217420PurposescQers promoter control #1 (low: minimal promoter, p027)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pre-associated oBC-mBC backbone (p025)
Pooled Library#194097Purposepre-associated oBC-mBC backbone (p025), can insert additional reporter constructsDepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only