We narrowed to 5,147 results for: codon optimized

1. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMD106h - intein-Cas9(C574)

   Plasmid

   #64191

   Purpose

   Expresses intein-Cas9(C574) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ALIX(P801G)-Strep

   Plasmid

   #180024

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX(1-868, P801G)-Strep (PDCD6IP Human)
   

   Tags

   Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   P801G

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO rtTA3-2A-Cre

   Plasmid

   #68448

   Purpose

   TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   rtTA3-2A-Cre
   

   Use

   Gmap

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in mice

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HCMVgL-FLAG (Merlin)

   Plasmid

   #136450

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein L (UL115 Human betaherpesvirus 5)
   

   Tags

   Short SGGSG linker and FLAG tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tt-NylC_pET28a

   Plasmid

   #215431

   Purpose

   Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Tt-NylC
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

   Plasmid

   #197961

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pco-vCasphi-2
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

    Plasmid

    #197962

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pco-nCasphi-2
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVRc03_1198

    Plasmid

    #49730

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS03_1198
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pscALPS-SIV-RCM vpx

    Plasmid

    #115812

    Purpose

    Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM Vpx (AF349680)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRa16_3622

    Plasmid

    #49670

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF16_3622
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1300_pUB10_pcoCASphi_E9t_MCS_version2

    Plasmid

    #197950

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscALPS-SIV-AGM-VER9063 vpr

    Plasmid

    #115825

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (Ver 9063)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sox17(2A-phiC31o)

    Plasmid

    #140762

    Purpose

    Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    phiC31o
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox17

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-R2ACR_142-EYFP

    Plasmid

    #103964

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R2ACR_142
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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