We narrowed to 5,092 results for: codon optimized

1. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pscALPS-SIV-RCM vpx

   Plasmid

   #115812

   Purpose

   Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVRCM Vpx (AF349680)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1300_pUB10_pcoCASphi_E9t_MCS_version2

   Plasmid

   #197950

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVRa16_3622

   Plasmid

   #49670

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF16_3622
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pscALPS-SIV-AGM-VER9063 vpr

   Plasmid

   #115825

   Purpose

   Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVAGM Vpr (Ver 9063)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sox17(2A-phiC31o)

   Plasmid

   #140762

   Purpose

   Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   phiC31o
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Endogenous Sox17

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-R2ACR_142-EYFP

   Plasmid

   #103964

   Purpose

   Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R2ACR_142
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKI-cjFOXL2-coemiRFP670-PGK-Puro

   Plasmid

   #186168

   Purpose

   Knock-in gene targeting in marmoset cells at FOXL2 locus

   Depositor

   Sho Yoshimatsu

   Article

   Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

   Insert

   emiRFP670
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-parkin W403A

    Plasmid

    #45974

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin W403A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid

    #70659

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-parkin C431S

    Plasmid

    #45976

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin C431S (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pscALPS-SIV-WRC-vpr

    Plasmid

    #115839

    Purpose

    Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVWRC Vpr (AM713177)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pscALPS-SIV-AGM-VERAGM3 vpr

    Plasmid

    #115826

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (Ver AGM3) (M30931)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pscALPS-SIV-SAB-92018 vpr

    Plasmid

    #115822

    Purpose

    Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVSAB Vpr (HQ378594)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRa15_436

    Plasmid

    #49667

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF15_436
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVRc11_987

    Plasmid

    #49733

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS11_987
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPS-SIV-MUS2-1246-vpr

    Plasmid

    #115841

    Purpose

    Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMUS21246 Vpr (EF070329)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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