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We narrowed to 3,974 results for: biorxiv

Showing: 2781 - 2800 of 3974 results

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mClover3-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-mTagBFP2-pA (JDW 1361)

Plasmid

#224535

Purpose

A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG-NLS-mTagBFP2-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME H2B mCerulean (JDW 1150)

Plasmid

#224543

Purpose

A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

H2B-mCerulean

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PDGFR-TMD (MTK 3B) (pZYW025)

Plasmid

#194218

Purpose

Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PDGFR TMD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry (MTK 4a) (pAN3844)

Plasmid

#194223

Purpose

Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

T2A-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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PGK Promoter (MTK 2) (pAN2822)

Plasmid

#194224

Purpose

Mammalian Toolkit part 2 encoding the PGK promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

Plasmid

#194229

Purpose

Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-LCB1-SynNotch-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm1

Plasmid

#222911

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 1 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm4

Plasmid

#222914

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm3

Plasmid

#222913

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 3 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

Plasmid

#222915

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm2

Plasmid

#222912

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 2 (NPM2 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Expression

Mutation

Promoter

EF1a, hU6, and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET)

Plasmid

#214083

Purpose

Mammalian expression tetramerization domain (TET) of human TP53

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

hP53DD (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-iPE2-Hygro

Plasmid

#214019

Purpose

inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

PE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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