Addgene

We narrowed to 5,442 results for: arl

Showing: 2781 - 2800 of 5442 results

Results per page:

Sort results by:

pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM189A

Plasmid

#218135

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM195A

Plasmid

#218125

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
M13 pIII

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM194A

Plasmid

#218124

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
luxAB

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_RV-B14_VP1

Plasmid

#202074

Purpose

Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

RV-B14 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_RV-A89_VP1

Plasmid

#202073

Purpose

Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

RV-A89 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_PV1_VP1

Plasmid

#202071

Purpose

Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

PV1 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_E30_VP1

Plasmid

#202070

Purpose

Bacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

E30 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_CVB1_VP1

Plasmid

#202069

Purpose

Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB1 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_CVA4_VP1

Plasmid

#202068

Purpose

Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVA4 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_FusionRed-SYFP2

Plasmid

#189760

Purpose

Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

FusionRed-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX1-VSVG-INDIANA2

Plasmid

#196623

Purpose

Express VSV-G protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

VSV-G

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Use

Tags

small GTPase (Rab5)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOPO-HqERV(del5'-LTR)

Plasmid

#164089

Purpose

transfection of Hq-ERV without 5'-LTR

Depositor

Maik Hintze

Article

Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

Insert

Hq-ERV

Use

Eukaryotic expression vector

Tags

Expression

Mutation

deletion of 5'-LTR

Promoter

none (5'-LTR deleted)

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOPO-HqERV(C1-20)

Plasmid

#164090

Purpose

transfection of Hq-ERV with light-inducible promoter

Depositor

Maik Hintze

Article

Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

Insert

Hq-ERV

Use

Eukaryotic expression vector

Tags

Expression

Mutation

5'-LTR replaced with light-regulatable C1-20…

Promoter

light-activatable C1-20

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP35-TD-HilA

Plasmid

#153332

Purpose

Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

HA4-7c12 Tandem Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP92-TD-HilA

Plasmid

#153331

Purpose

Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP92-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge