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Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
UseTagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)
Plasmid#65446PurposeExpression of ephrinB1 and RFP in mammalian cellsDepositorInsertephrin B1 (Efnb1 Mouse)
UseLentiviralTagsSV40-RFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-fCX3CL1
Plasmid#164421PurposeAAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptorsDepositorInsertCx3cl1 (Cx3cl1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhuman red opsinAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V3-CD20-Puro
Plasmid#209757PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMminiSOX
Plasmid#206383PurposeExpresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MAFF
Plasmid#192917PurposeBarcoded piggybac transposon vector with Dox-inducible expression of MAFFDepositorInsertMAFF (MAFF Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB2-SV40-GFP (B2G)
Plasmid#65442PurposeExpression of mouse EPHB2 and GFP in mammalian cellsDepositorInsertEPHB2 (Ephb2 Mouse)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationSee depositor comments below.PromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-Best1-sCX3CL1
Plasmid#164422PurposeAAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epitheliumDepositorInsertCx3cl1 (Cx3cl1 Mouse)
UseAAVTagsExpressionMammalianMutationdeleted terminal 59 amino acidsPromoterBest1Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationbromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIPdU3-MND-FLAG-FLI1-IRES-GFP
Plasmid#129496PurposeLentiviral expression of human FLI1DepositorInsertFLI1 (FLI1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterMNDAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2433
Plasmid#144027PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID1A (ARID1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgArid1a#2/Cre
Plasmid#173572PurposeExpresses a Arid1a-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Arid1a (Arid1a Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgArid1a#1/Cre
Plasmid#173571PurposeExpresses a Arid1a-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Arid1a (Arid1a Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75120PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4DepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-329
Plasmid#140146PurposeGAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4DepositorInsertKLF4 Kruppel factor (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 330 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
Plasmid#139444PurposeLentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4DepositorInsertFLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsequence codon-optimizedPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-156
Plasmid#140147PurposeGAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)DepositorInsertKLF4 aa 1-156 (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 157 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
Plasmid#75116PurposeRetroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutationDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationsingle amino acid mutation N216APromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK2 gRNA (BRDN0001146193)
Plasmid#76214Purpose3rd generation lentiviral gRNA plasmid targeting human NEK2DepositorInsertNEK2 (NEK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only