Addgene

We narrowed to 2,791 results for: nicola

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pEPQD0CM0061

Plasmid

#177026

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26

Use

Synthetic Biology

Tags

Expression

Mutation

amplified from cDNA, contains T359S relative to K…

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0060

Plasmid

#177024

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0059

Plasmid

#177023

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0063

Plasmid

#177021

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0065

Plasmid

#177019

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed, amplified from cDNA, con…

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0173

Plasmid

#154640

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s (ΔAP2+1) Firefly luciferase expression cassette [35s (ΔAP2+1)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0332

Plasmid

#162314

Purpose

Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

SP6prom-EO1-sfGFP-HiBiT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CB0026

Plasmid

#162285

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CB0246

Plasmid

#162286

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0296

Plasmid

#162305

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, superfolder GFP, no stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQDKN0329

Plasmid

#162315

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease-HiBiT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0027

Plasmid

#162309

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0548

Plasmid

#162299

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-TrxA-[TEV cleavage site]

Use

Synthetic Biology

Tags

Expression

Mutation

codon optimized for E. coli

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0549

Plasmid

#162300

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, SUMO-[TEV cleavage site]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0550

Plasmid

#162301

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-SUMO-[TEV cleavage site]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC0CM0258

Plasmid

#162291

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0283

Plasmid

#162288

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge