-
Plasmid#186760PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc4
Plasmid#186761PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc5
Plasmid#186762PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc2_STOP
Plasmid#186767PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.DepositorInsertNanoluciferase
UseTagsExpressionMutationPremature stop codon at residue 16PromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sGluc
Plasmid#186769PurposeAn mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.DepositorInsertGaussia luciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TK-GFP-Nluc-P2A-neo-TK
Plasmid#134896PurposeExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locusDepositorInsertTK-GFP-Nluc-P2A-Neo-TK
UseCryptosporidium expressionTagsExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIRCgp41-1
Plasmid#74227PurposeVector for SICLOPPS-mediated protein circularization using the gp41-1 intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsgp41-1 C-intein and gp41-1 N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::H2B-mScarlet
Plasmid#186764PurposeA plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.DepositorInsertH2B-mScarlet
UseOverexpressionTagsH2BExpressionMutationPromoterGAPDHAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF2
Plasmid#186766PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIRCNpu
Plasmid#74226PurposeVector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsNpu DnaE C-intein and Npu DnaE N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
-
pTNFR1-tdEOS
Plasmid#98273Purposemammalian expression of TNFR1-tdEOSDepositorInsertTNFR1 (TNFRSF1A Human)
UseTagsFlag and tdEosExpressionMammalianMutationPromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgTK
Plasmid#122852PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK geneDepositorInsertsCas9-GFP
U6-sgTK
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPExpressionMutationPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPExpressionMutationPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
UPRT-mCh-Nluc-P2A-neo-UPRT
Plasmid#135015PurposeExpresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locusDepositorInsertsmCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR
UseCryptosporidium expressionTagsmCherry and Nluc-neoExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SFFV-GLuc-CreERT2-mCherry
Plasmid#178185Purposethe plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.DepositorInsertsGLuc
CreERT2
mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xNFkB Luc
Plasmid#111216Purposereporter geneDepositorInsertfirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA52_Lenti.puro.U6_modified DR
Plasmid#196723PurposeFor cloning pairs of AsCas12a guides with two DRs that are as different as possible.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BFP-U6-sgRNA
Plasmid#196725PurposeSortable sgRNA expression, Chen tracrRNA PMID: 24360272DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only