Addgene

We narrowed to 23,228 results for: CHI

Showing: 651 - 700 of 23228 results

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pVITRO1-BFP-NLS

Plasmid

#184458

Purpose

Nucleus-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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3ME6/mSprouty2

Plasmid

#22097

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (Spry2 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pVITRO1-iRFP702-LaminB1

Plasmid

#184456

Purpose

Nuclear membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-NES

Plasmid

#184449

Purpose

Cytosolic TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCherry-CAAX

Plasmid

#186347

Purpose

Plasma membrane-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCherry-NLS

Plasmid

#186346

Purpose

Nucleus-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-iRFP702-NLS

Plasmid

#184454

Purpose

Nucleus-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-iRFP702-CAAX

Plasmid

#184455

Purpose

Plasma membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-iRFP702-NES

Plasmid

#184457

Purpose

Cytosolic iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
3CB1/cSprouty2

Plasmid

#22096

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (SPRY2 Chicken)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
RS314-U6(PolII-Lsm)

Plasmid

#200772

Purpose

Pol II expression of U6 Lsm

Depositor

Aaron Hoskins

Article

Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

Insert

Gal-SNR6-II

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-LaminB1

Plasmid

#184448

Purpose

Nuclear memebrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-TagRFP-NLS

Plasmid

#184446

Purpose

Nucleus-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCherry-LaminB1

Plasmid

#186348

Purpose

Nuclear membrane-targeted mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCardinal-NES

Plasmid

#184453

Purpose

Cytosolic mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-TagRFP-CAAX

Plasmid

#184447

Purpose

Plasma membrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCardinal-NLS

Plasmid

#184450

Purpose

Nucleus-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCardinal-LaminB1

Plasmid

#184452

Purpose

Nuclear memebrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-BFP-NES

Plasmid

#184461

Purpose

Cytosolic EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bactin2:loxP-STOP-loxP-DsRed-express

Plasmid

#68797

Purpose

For making transgenic fish

Depositor

David Traver

Article

Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

Inserts

beta-actin promoter
loxp-stop-codon-loxp-dsred

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/Sp6

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR-mEGFP

Plasmid

#203774

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Csy4-PE2

Plasmid

#172667

Purpose

Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

Csy4-PE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7/Sp6

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-BclXL-pEGFP-C1

Plasmid

#177408

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
psgRNA Library

Pooled Library

#115927

Purpose

Genome-wide knockdown of E. coli genes

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Expression

Bacterial

Species

Escherichia coli

Use

CRISPR

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only
psgRNA_EcoWG1

Pooled Library

#131625

Purpose

Improved genome-wide knockdown of E. coli genes

Depositor

David Bikard

Article

Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

Expression

Bacterial

Species

Escherichia coli

Use

CRISPR

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only
T7-VEE-GFP

Plasmid

#58977

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
SP6-VEE-GFP

Plasmid

#58976

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p3HA-hMIB2

Plasmid

#33312

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB2 (MIB2 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172668

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

ALDOB pegRNA and ALDOB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172669

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

RNF2 pegRNA and RNF2_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
S. rosetta Col+ cDNA amplified library

Pooled Library

#62844

Purpose

amplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensis

Depositor

Nicole King

Article

Fairclough et al Genome Biol. 2013 Feb 18;14(2):R15. doi:

Expression

Bacterial

Species

Salpingoeca rosetta

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-tBid-pEGFP-C1

Plasmid

#166736

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

tBid (Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3F-Strep-mMALT1

Plasmid

#33315

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Malt1 (Malt1 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
plenti6.3/hCx43-stop

Plasmid

#27383

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Gja1 (GJA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
Venus-Bad-pEGFP-C1

Plasmid

#166733

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bad (BAD Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3F-Strep-mBCL10

Plasmid

#33314

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

P-TIGHT

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Showing: 651 - 700 of 23228 results

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