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Plasmid#117752PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-1-NONO(53-312)
Plasmid#135442PurposeExpresses NONO 53-312 in the second site; for co-expression with His-tagged proteinDepositorInsertNon-POU domain-containing octamer-binding protein (NONO Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
3521 pET15b Bid p15
Plasmid#8782DepositorInsertBid p15 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses the Gluc-iLID fusion protein in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET 15b-UbcH5a/2417
Plasmid#11472DepositorInsertUbcH5a (UBE2D1 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-his6-HA3-CLIP
Plasmid#188942PurposeT7 expression vector for making his6- and HA3-tagged CLIP proteinDepositorInserthis6-HA3-CLIP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-KIX (586-672)
Plasmid#99336PurposeBacterial expression of mouse CBP KIX domainDepositorInsertCBP KIX (586-672) (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.1
Plasmid#193802PurposeProtein production of ChemoG3.1 in bacteriaDepositorInsertChemoG 3.1
UseTags10xHisExpressionBacterialMutationEGFP: A206K, T225RPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.2
Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
UseTags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO NL-FKBP-Cas9
Plasmid#162730PurposeBacterial expression, protein expressionDepositorInsertFKBP F36V
UseTags6xHis and SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorInsertMed25-VBD (MED25 Human)
UseTagsExpressionBacterialMutationinsert is 394-543 onlyPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorInsertLanCL2 (LANCL2 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only