pET-EIN-FTO(32-505)
(Plasmid #186466)

• Purpose: Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
• Depositing Lab: Vincenzo Venditti
• Publication: Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:105540. doi: 10.1016/j.pep.2019.105540.

Backbone

• Vector backbone: pET (21a)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 6222
• Total vector size (bp): 7644
• Modifications to backbone: The size is 6Histidine+EIN+ Pet21a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fat mass obesity associated protein
• Alt name: FTO
• Species: H. sapiens (human)
• Insert Size (bp): 1421
• Mutation: amino acid 32-505
• GenBank ID:
• Entrez Gene: FTO (a.k.a. ALKBH9, BMIQ14, GDFD, IFEX9)
• Promoter: T7
• Tag / Fusion Protein:
  • 6 Histidine-EIN (N-terminal domain of E.coli Enzyme I) (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTTCAGGCATTTTAGCAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The article where this gene was cloned is as follows- https://pubmed.ncbi.nlm.nih.gov/31740367/
This gene is similar to another addgene deposit (Number 125732) instead of Alkbh5 sequence FTO sequence is inserted.