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pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
Plasmid#56658Purposeexpresses constitutively active human PKCi with a deletion on the PB1 domainDepositorInsertHuman PKCi (PRKCI Human)
UseLentiviralTagsV5/HisExpressionMutationA120E and deletion of PB1 domain (aa 24–98)PromoterCMVAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(N863A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation20 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I
Plasmid#146553PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorInsertHsSmg6-del36-576-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaP)
Plasmid#83507PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationdelta . Deletion of residues 187-283PromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaC)
Plasmid#83506PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationdelta C. Contains residues 1-339PromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 merlin 308-590
Plasmid#11627DepositorInsertmerlin (NF2 Human)
UseTagsFlagExpressionMammalianMutationCarboxy-terminal half of merlin isoform 2.PromoterAvailable sinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)
Plasmid#159658PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)
Plasmid#159657PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPFAS-2XStrep
Plasmid#125682Purposeexpresses human PFAS protein with a C-terminal 2XStrep affinity tagDepositorInsertPFAS (PFAS Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS61APromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
peGFPC2-CARKL
Plasmid#51748Purposeexpression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK)DepositorInsertSHPK (Shpk Mouse)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Flag-TRIM24-C840W shRescue
Plasmid#28137DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationC840Wmutated at aa 308 and 309 for shRNA rescue; …PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXPR_sgGFP3
Plasmid#119875PurposegRNA expression vectorDepositorInsertsgGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc
Plasmid#129105PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone productionDepositorInsertBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCarCBAE
Plasmid#117755PurposeCodon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE223 human myc-Chibby-pCS2+MT
Plasmid#16825DepositorInsertmyc-Chibby (CBY1 Human)
UseXenopus expressionTags6-mycExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only
