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Showing: 2741 - 2760 of 8855 results

  1. KP401: pMVP (L5-L4) eCFP-P2A

    Plasmid
    #121712
    Purpose
    pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    eCFP-P2A (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121712-plasmid-map-sequence-id-237065

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  5. pJB03

    Plasmid
    #111961
    Purpose
    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Use
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    Tac
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111961-plasmid-map-sequence-id-219904

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  6. pBad-CA TAG186

    Plasmid
    #105837
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 186
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105837-plasmid-map-sequence-id-205518

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  7. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  15. pJK381

    Plasmid
    #71573
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to glycine
    Promoter
    recA
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71573-plasmid-map-sequence-id-140454

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  16. pJK382

    Plasmid
    #71574
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to asparagine; silent mutat…
    Promoter
    recA
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71574-plasmid-map-sequence-id-130942

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  17. pJK347

    Plasmid
    #71833
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate
    Promoter
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71833-plasmid-map-sequence-id-131050

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  18. pJK348

    Plasmid
    #71832
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine
    Promoter
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71832-plasmid-map-sequence-id-131051

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  19. pJK193

    Plasmid
    #71834
    Purpose
    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71834-plasmid-map-sequence-id-131053

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Showing: 2741 - 2760 of 8855 results