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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,777 results for: plat

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Showing: 2741 - 2760 of 8777 results

  3. GluN2B/NR2B glutamate receptor scFv [N59/20]

    Plasmid
    #182092
    Purpose
    Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.
    Depositor
    James Trimmer
    Publication
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)
    Tags
    Sortase, His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182092-plasmid-map-sequence-id-357627

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  4. Anti-LRRK2/Dardarin, C-terminus [N241A/34R]

    Plasmid
    #177520
    Purpose
    Mammalian Expression Plasmid of anti-LRRK2/Dardarin, C-terminus (Human). Derived from hybridoma N241A/34.
    Depositor
    James Trimmer
    Publication
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-LRRK2/Dardarin, C-terminus (Homo sapiens) recombinant mouse monoclonal antibody (LRRK2 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177520-plasmid-map-sequence-id-348942

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  5. SOX2 KO (T2A-H2B-EGFP)

    Plasmid
    #167969
    Purpose
    SOX2 CDS replacement with T2A-H2B-EGFP
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRY-Box Transcription Factor 2 (SOX2 Human)
    Use
    CRISPR; Donor template
    Tags
    H2B-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167969-plasmid-map-sequence-id-331127

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  7. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid
    #211776
    Purpose
    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Traptavudin-DNMT3A
    Tags
    3xV5
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211776-plasmid-map-sequence-id-417370

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  8. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  9. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ie1-GFPc
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  10. pJAT62

    Plasmid
    #204301
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204301-plasmid-map-sequence-id-405845

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  11. pODN-mR2-ACTB

    Plasmid
    #183868
    Purpose
    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB homology arms with mRuby2-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183868-plasmid-map-sequence-id-362451

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  12. pODN-mNG-RHOA

    Plasmid
    #183836
    Purpose
    Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RHOA homology arms with mNeonGreen-linker (RHOA Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183836-plasmid-map-sequence-id-362444

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  15. CFP-GIV-CT WT

    Plasmid
    #69532
    Purpose
    Mammalian expression of The CFP-tagged GIV-CT biosensors
    Depositor
    Pradipta Ghosh
    Publication
    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GIV (CCDC88A Human)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    C terminal amino acids S1660-S1870 of human GIV
    Promoter
    CMV
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    69532-plasmid-map-sequence-id-127354

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  17. pCEP4-myc-PDGFRalpha-V242K

    Plasmid
    #136436
    Purpose
    Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    V242K (disrupts PDGF binding, but still binds HCM…
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136436-plasmid-map-sequence-id-263246

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  18. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid
    #107323
    Purpose
    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107323-plasmid-map-sequence-id-204717

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Showing: 2741 - 2760 of 8777 results