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Showing: 2741 - 2760 of 3500 results

  1. pcDNA5-LAP-KNL1-M3

    Plasmid
    #115896
    Purpose
    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.
    Depositor
    Geert Kops
    Article
    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.
    Insert
    KNL1-M3 (KNL1 Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Fragment of KNL1 containing three KNL1 repeat seq…
    Promoter
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    115896-plasmid-map-sequence-id-224847

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  2. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

    Plasmid
    #74606
    Purpose
    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Y101A, Y103A, W120A
    Promoter
    SFFV
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74606-plasmid-map-sequence-id-151888

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  3. pGEX6P1-human LIC1 C-terminal half

    Plasmid
    #74599
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    T7
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74599-plasmid-map-sequence-id-145961

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  4. TFORF0959

    Plasmid
    #141766
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ERF (ERF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141766-plasmid-map-sequence-id-388527

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  9. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid
    #72922
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    TRE tight
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72922-plasmid-map-sequence-id-138771

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  19. pCHMP2A-mut-NS3-Green

    Plasmid
    #223529
    Purpose
    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing a mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP2A (CHMP2A Human)
    Use
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223529-plasmid-map-sequence-id-443350

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  20. GPBA-DuET

    Plasmid
    #213255
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPBA (GPBAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213255-plasmid-map-sequence-id-430805

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Showing: 2741 - 2760 of 3500 results