Addgene

We narrowed to 17,270 results for: gateway

Showing: 2721 - 2740 of 17270 results

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pDONR223 LC3A G120A

Plasmid

#123070

Purpose

Gateway entry clone encoding human MAP1LC3A G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBEG R1-ChlorR-R4 #BGV049

Plasmid

#48943

Purpose

Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

na

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
IG301: pMVP (L1-R5) CMV promoter

Plasmid

#121692

Purpose

pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A

Plasmid

#123073

Purpose

Gateway entry clone encoding human MAP1LC3B G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 G116A

Plasmid

#123087

Purpose

Gateway entry clone encoding human GABARAPL2 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 116 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP no-stop

Plasmid

#123209

Purpose

Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C no-stop

Plasmid

#123208

Purpose

Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IF701: pMVP (L5-L4) APEX2

Plasmid

#121725

Purpose

pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR-ARF19

Plasmid

#83846

Purpose

Gateway donor vector for ARF19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

ARF19 (ARF19 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARF6

Plasmid

#67314

Purpose

hArf6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 GABARAPL1 G116A

Plasmid

#123084

Purpose

Gateway entry clone encoding human GABARAPL1 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 116 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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HE401: pMVP (L1-R5) shRNA

Plasmid

#121698

Purpose

pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj11

Plasmid

#173139

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj11 (kcnj11 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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JZ901: pMVP (L5-L4) EF1a promoter

Plasmid

#121727

Purpose

pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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