-
Plasmid#154450PurposeExpression of FLAG/HA-tagged HDLBPDepositorInsertHDLBP (HDLBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-wtGATA6-3XAU1 puro
Plasmid#72607PurposeExpresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cellsDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EA2, R57A, R59A pMT2
Plasmid#206122Purposeexpression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currentsDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationCav2.1-P1217fs, R57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-actin-pcDNA4-TO
Plasmid#118370PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.DepositorInsertactin beta (ACTB Human)
UseTagsHA-GFP1-10ExpressionMammalianMutationPromoterCMV/TOAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-wtGATA6-3XAU1 neo
Plasmid#72618PurposeExpresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE TightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-ACTB_sgRNA
Plasmid#183884PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H513 px330.sgActin.UTR.2
Plasmid#138176PurposeCRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.DepositorInsertsgActin (Actb Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Actin-C-18
Plasmid#56151PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorInsertNM_001101.3 (ACTB Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE N' EGFP NAP1 FL IRES Blast
Plasmid#179100PurposeExpression of full length NAP1 cDNADepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcdna6.2- myc ULK1 4SA
Plasmid#27631DepositorInsertmouse myc ULK1 4SA (Myc Mouse)
UseTagsmycExpressionMammalianMutationS467A, S555A, T574A, S637A. Initial M from ULK1 r…PromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst-mBMP7
Plasmid#127375PurposeAllows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-mEGFP
Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP (ACTB Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3543
Plasmid#145019PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCSDC2 (CSDC2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsNoneExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 wt
Plasmid#27626DepositorInsertmouse myc ULK1 (Myc Mouse)
UseRetroviralTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A
Plasmid#74605Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-Actin-C-18
Plasmid#54863PurposeLocalization: Actin, Excitation: 568, Emission: 592DepositorInsertActin (ACTB Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cntn1-AP-His
Plasmid#71939PurposeExpresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn1 (Cntn1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARΔ142–337
Plasmid#89106PurposeMammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1DepositorInserthAR delta 142-337 (AR Human)
UseTagsExpressionMammalianMutationhAR delta 142-337PromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only