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Plasmid#187459PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC-N-GFP
Plasmid#172974PurposeMAC-tagged GFP expressionDepositorInsertGFP
UseTagsMAC-tagExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
BK beta1 EGFP
Plasmid#113557Purposemouse BK beta1 subunit in C-terminal translational frame with EGFPDepositorInsertKcnmb1 (Kcnmb1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-RB 1-602
Plasmid#16006DepositorInsertRb (RB1 Human)
UseTagsEGFPExpressionMammalianMutationAmino acids 1-602PromoterAvailable sinceJune 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1d
Plasmid#134906PurposeExpression of rat Myo 1d (Myr 4) in mammalian cells.DepositorInsertMyo 1d (rat) with 3' UTR (Myo1d Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP48-2A(sfGFP)
Plasmid#126993PurposeLevel 2 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSGFPnERT(#205)
Plasmid#184065Purposecyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInserteGFP-nls-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA2[PLac:GFP]
Plasmid#60778PurposeContains Plac driving expression of GFPDepositorInsertyemGFP
UseTagsExpressionMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-QL
Plasmid#86076PurposeA mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationChanged glutamine 67 to leucinePromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGFP FRNK
Plasmid#50518PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, mutated
UseTagsGFPExpressionMammalianMutationtruncated (NT2186-3268)PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-TYK2
Plasmid#139354PurposeExpression of TYK2 protein fused with GFPDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SAGFP
Plasmid#127100PurposeSA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.DepositorInsertSA-GFP reporter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
Plasmid#209282PurposeExpresses FR-GECO1c and CoChR in the zebrafish heartDepositorInsertFR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
UseZebrafish expressionTagsExpressionMutationPromoterzACNE20_myl7Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
8047_GFP-Kras2B_ires_puro
Plasmid#64371PurposeThis a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 WT
Plasmid#74950Purposemammalian expression of Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOBCol2.3-GFPemd
Plasmid#110210PurposeLabels cells in the late osteoblast lineage.DepositorInserteGFPemerald
UseTagsNoneExpressionMammalianMutationPromoter2.3 kb rat type 1 collagen + first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-5'dGFP3'
Plasmid#26425DepositorInsert5'dGFP3'
UseTagsCamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…ExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
mTagGFP2-Clathrin-15
Plasmid#56413PurposeLocalization: Clathrin Vesicles, Excitation: 483, Emission: 506DepositorInsertClathrin (CLTB Human)
UseTagsmTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p274 EF1a_EGFP_hH11
Plasmid#164851PurposeEGFP-expression vector for human H11 locusDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only