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Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
CH00X
Plasmid#73517PurposeTagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-TDepositorInsertpmex-5::TagRFP-T::PH::tbb-2
UseTagsTagRFP-TExpressionMutationPromotermes-5Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM28521
Plasmid#105359PurposeSynthetic biologyDepositorInsertEC1.2en-EC1.1p fusion promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wElectra1
Plasmid#184932PurposeCodon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmidDepositorInsertElectra1
UseTagsExpressionWormMutationPromotertag-168Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO mouse ULK2 shRNA 93
Plasmid#27635DepositorInsertmouse ULK2 shRNA 93
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
tph-1(short)::rpl-22-HA
Plasmid#122203PurposeC. elegans tagged ribosome expression plasmidDepositorInserttph-1(short)::rpl-22-HA
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NPY-td-Orange2
Plasmid#83497PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorInsertNPY (NPY Human)
UseTagstandem mOrange2ExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_W267N
Plasmid#214311PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationW267NPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-v5.9
Plasmid#155259PurposePlasmid for the aTc-inducible expression of the v5.9DepositorInsertv5.9
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
Plasmid#66753PurposeminiSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondriaDepositorInsertminiSOG
UseTagstomm-20 (N terminal 55 aa)ExpressionWormMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 T183AY185F
Plasmid#118299PurposeExpression of Rat ERK2DepositorInsertERK2 T183AY185F (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationT183AY185FPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAK112
Plasmid#167280PurposeCodA-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB34
Plasmid#122245PurposeLentivirus delivery for stable expression of LbCas12a-VPRDepositorInsertmammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorExpressionMutationPromoterEFsAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMB22
Plasmid#122244PurposeLentivirus delivery for stable expression of AsCas12a-VPRDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorExpressionMutationPromoterEFsAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 K52R
Plasmid#118298PurposeExpression of Rat ERK2DepositorInsertERK2 K52R (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationK52RPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only