Addgene

We narrowed to 11,140 results for: 110

Showing: 2681 - 2700 of 11140 results

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pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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negMaLionG

Plasmid

#113907

Purpose

negative control for Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Use

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual- GST-TEV-TBK1

Plasmid

#208875

Purpose

For expression in insect cells of TBK1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-c-myc-zeo

Plasmid

#17758

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

c-myc (MYC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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ICP8-P2A/Crimson (pSLIK4)

Plasmid

#113859

Purpose

For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3A

Plasmid

#13742

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3A (Kif3a Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-NMHC II-B

Plasmid

#11348

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-B (MYH10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2006

Availability

Academic Institutions and Nonprofits only

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Article

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Insert

MTTP (MTTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pNICE-LAP-neurexin-1beta

Plasmid

#42575

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LAP-neurexin-1beta

Use

Tags

c-Myc, after signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pET24b-Amg2

Plasmid

#199434

Purpose

expresses Amg2 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

Amg2

Use

Tags

his tag

Expression

Bacterial

Mutation

L159K mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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MaLionB

Plasmid

#113910

Purpose

Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(T17N)

Plasmid

#13721

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(T17N) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Use

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

Plasmid

#214998

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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