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Plasmid#193648PurposeExpression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-rAPOBEC1, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-CDA1
Plasmid#193649PurposeExpression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-PmCDA1, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-eAID
Plasmid#193650PurposeExpression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-eAID, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSDT (JBEI-11535)
Plasmid#87934PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDTDepositorUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_fibrillarin
Plasmid#65710PurposeExpresses ONL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_fibrillarin
Plasmid#65708PurposeExpresses YNL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry-IRES-Cre
Plasmid#55632PurposeExpresses Cre in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV8InsertsmCherry
Cre
UseAAVTagsExpressionMammalianMutationPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Flpo
Plasmid#55634PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV1InsertsmCherry
Flpo
UseAAVTagsExpressionMammalianMutationPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorInsertsUseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGES401
Plasmid#190198PurposeFor CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-WT
Plasmid#25642DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralTagsExpressionMammalianMutationAn NsiI restriction site was engineered into the …PromoterAvailable sinceApril 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE8e-V106W
Plasmid#193646PurposeExpression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationTadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-ABE8e-V106W
Plasmid#193656PurposeExpression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, TadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-Tq2CFP-OcsT
Plasmid#71268PurposeEntry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTq2CFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194885PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA1and2
UseLentiviralTagsHAExpressionMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-dDIO hChR2(H134R)-EYFP
Plasmid#55640PurposeDre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only