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pLentiCRISPRv2 sgSp1
Plasmid#174907PurposeSecond generation sgRNA against Sp1DepositorInsertSpecificity Protein 1 (SP1 Human)
UseTagsExpressionMammalianMutationMissense mutation to mutate PAM sequencePromoterEFS-NSAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_TNF-SBP-mCherry
Plasmid#65283PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-EGFP_puromycin
Plasmid#65284PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1F+2
Plasmid#190833PurposeFor mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD
Plasmid#197687Purposeplasmid for expressing the DNMT3bCDDepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 MT
Plasmid#78259PurposepsiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated miR-34 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CCGT to GG…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-mCherry
Plasmid#65259Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(1-555)
Plasmid#90105PurposeMammalian expression of Flag-MCPIP1(1-555)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-PPxY
Plasmid#61006PurposeExpresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 PPxY mutation (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPPxY mutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-FL-wt
Plasmid#31621DepositorInsertClaspin (CLSPN Human)
UseTagsFlag, GST, and HisExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF5
Plasmid#58315PurposeHA-tagged mouse TRAF5DepositorInsertTNF receptor associated factor 5 (Traf5 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-del-N
Plasmid#61004PurposeExpresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 delete N (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationdeleted amino acids 17-310PromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human MOB1
Plasmid#37024DepositorInsertMOB1B (MOB1B Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-del-C
Plasmid#61005PurposeExpresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 delete C (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationdeleted amino acids 934-1187PromoterCMVAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP_mEGFP-GluA1c
Plasmid#114136PurposeMammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.DepositorInsertmEGFP tagged GluA1 C-terminus
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta
Plasmid#33322DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationPromoterhuman PGKAvailable sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only
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